Instruments for NMR Spectroscopy
NuMega operates two 500 MHz Bruker NMR spectrometers, DRX 500 and AV-500. We have a variety of highly sensitive probes that can be interchangably fitted into our magnet. The diversity of both instruments enables us to carry out various experiments with a fast turnaround time. We perform the following experiments:
- 1D- H-1, C-13, P-31 and other nuclei, such as B-10, F19, Si-29, V-51, Sn-119, etc.
- 1D NOE, Water Supression, Decoupling, VT, Quantitative NMR, DEPT, DEPTQ, INEPT
- 2D-COSY, NOESY, TOCSY, ROESY, HSQC, HMBC, and other pulse sequences.
- We also design NMR experiments, develop test methods and interpret your data. If you want us to perform an experiment not listed, please contact us to see how we can assist you.
NMR Probes Available at NuMega
- Inverse Probe: 5mm, Triple Channel, High Sensitive H-1, Pulsed Field Gradient Coil
- Broad Band Probe: 5 mm, Capable of being tuned to P-31, C-13, N-15, B-10, B-11, V-51, Sn-119, 0-17, Li-7, etc., Pulsed Field Gradient Coil
- Broad Band Probe: 10mm, 4x volume-strong signal
Instruments for Mass Spectroscopy
NuMega operates a Perkin Elmer PE-SCIEX API-150 mass spectrometer equipped with an electrospray ionization source. Electrospray, developed in 1980s, is a mild ionization technique, which is useful for nonvolatile and unstable materials. Normally the parent ions are the dominant signals. Positive mode usually detects M+H+, M+Na+, and M+K+, while negative mode detects M+H-, and M+Cl-. Our detection range on the instrument is 30 – 3000 m/z. We report both the positive and negative ionization scans of your sample.
Instruments for Elemental Analysis
NuMega operates a Perkin Elmer PE2400-Series II, CHNS/O analyzer for elemental analysis. The CHNS content is determined using the Pregl-Dumas method where your sample is prepared, introduced into the combustion chamber and burned at high temperature 925°F under pure oxygen. The resulting gas mixture and helium carrier gas pass through various reductive and catalytic zones to convert the gas mixture into CO2, H2O, N2, and SO2. The gasses are separated using gas chromatography and quantified with a thermal conductivity detector.